We will present surface enhanced Raman spectroscopy (SERS) detection of molecules using plasmonic nanoparticles that are embedded in agarose gel and in filter paper-integrated microfluidic channels, respectively. It has been known that, when SERS detection is performed in complex fluids such as cell culture media, a method to reduce interferences from a variety of molecules in the fluids on the detection results is very important. If continuous monitoring of molecules in cell culture media is needed, there should be a method to prevent large molecules such as proteins from reaching SERS substrates when sample solutions flow over the substrates. Since both agarose gel and filter paper can be used to separate molecules by size, in this study we have integrated them with plasmonic nanoparticles for SERS detection in complex fluids. We will report how to use filter paper-integrated microfluidic channels to detect melamine and sodium thiocyanate (NaSCN) in milk using SERS. In addition, we will demonstrate how to use plasmonic agarose gels to detect illegal drug in urine.
We will present surface enhanced Raman spectroscopy (SERS) and localized surface plasmon resonance (LSPR) detection of hydrogen peroxide (H2O2) using plasmonic gels. It has been known that reactive oxygen species (ROS), such as hydrogen peroxide, are involved in various biological processes, including metabolism, cell signaling, protein folding, biosynthesis, and host defense. Therefore, developing a simple and sensitive method for monitoring ROS levels is very important for live cell studies. Nevertheless, a challenge of utilizing SERS-based or LSPR-based method for molecular detection in complex fluids, such as cell culture media, is that a variety of molecules in sample solutions could interfere with detection results. In addition, when using SERS-based methods, the chance of having the target molecules at the SERS hot spots is reduced when other molecules are present in the solution. To enable detection of H2O2 in cell culture media, we have developed SERS and LSPR detection methods based on gels containing plasmonic nanoparticles. Since gels are filter-like materials, H2O2 can penetrate through the gels, but cells and large molecules such as proteins are blocked. We have successfully utilized these two methods to detect H2O2 in cell culture media without any sample pretreatment.
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