Mr. Yunqing Hao Profile

Yunqing Hao

at Nanjing Univ

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Proceedings Article | 22 February 2019 Paper

Development of an electrothermal MEMS mirror based two-photon microscopy probe

Liang Zhou, Yanpin Chen, Xiaohong Chen, Yunqi Hao, Jason Coleman, Huikai Xie

Proceedings Volume 10882, 1088230 (2019) https://doi.org/10.1117/12.2507655

KEYWORDS: Actuators, Two photon excitation microscopy, MEMS based endomicroscopy

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Two-photon microscopy (TPM) has shown its great impact in studying neuronal activity of live animals. Commercial TPM systems require the animals under study to be restrained under the microscope and the 3D images are obtained typically by using bulky galvo scanning mirrors, which limits the flexibility of animal brain imaging to a large extent. To study freely moving animals, miniaturization of the optical scanning system is the key. In this work, a miniature TPM probe has been developed based on an electrothermal MEMS mirror that can be driven under low voltage. The MEMS mirror has an initial tilt angle after fabrication, and its footprint is 3 mm x 4 mm and reflective mirror plate is aluminumcoated with an equivalent diameter of 2.5 mm. This MEMS probe can be directly adapted to a commercial TPM system. In addition to the MEMS mirror for laser beam scanning, inside the MEMS probe head there are a fixed mirror to fold the laser beam and a high NA polymer lens for focusing. This miniature probe can realize an FOV of 3.5°, or a scanning range of 150 μm. This MEMS probe head is compact with a size of 8 mm x 16 mm, which can be further scaled down.

Proceedings Article | 30 October 2009 Paper

The dynamics of land cover change pattern and landscape fragmentation in Jiuzhaigou Nature Reserve, China

Yunqing Hao, Jinxi Wang, Hong Jiang

Proceedings Volume 7498, 74983P (2009) https://doi.org/10.1117/12.830050

KEYWORDS: Vegetation, Stereolithography, Ecosystems, Multispectral imaging, Climatology, Remote sensing, Climate change, Animal model studies, Earth observing sensors, Landsat

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Nature reserves are facing a very challenging conflict between conservation and development today. The protection effect of Jiuzhaigou Nature Reserve has received considerable attention, especially in view of its stimulating tourism after the 1990s. Remote sensing data from 1974, 1994 and 2002 were chosen for the analysis of this reserve, since they closely represented the vegetation situation before and after felling, as well as the disturbance from the flush of tourism. We find that the quality and quantity of the forest in Jiuzhaigou Nature Reserve continues to decline, owing to the conifer area and whole forest area constantly shrinking, as well as the landscape fragmentation increasing. The rate of loss of forest in the second period (1994-2002) had showed down much than that in the first period (1974-1994), due to nature protection. Shrubland area continued to increase throughout the two periods, with an increased speed in the second period which was about 3.5 times that in the first period. The treeline was consistently retreating, contributing a total of 467.96 ha of conifer forest lost. However, the causes involved in tree mortality and the resulting regression of the treeline are not clear. In any case, under the general background of global warming, human impact undoubtedly should have direct or indirect cause dramatic consequences for the forest in this very sensitive zone, while there are many uncertainties in the behaviour of high mountain ecosystems. Keywords: Jiuzhaigou Nature Reserve, Vegetation, Habitats fragment,

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