The structural and functional imaging of ophthalmic tissues in cellular level play an important role in the understanding and evaluation of the physiology and pathology of ophthalmic diseases. In this study, we developed a dual-mode full-field optical coherence tomography (FFOCT) that is capable of acquiring label-free cellular images of freshly excised ophthalmic tissues, achieving static contrasts gained from structural refractive index gradients and dynamic contrast induced by endogenous cell motility related to cell functions. Through imaging experiments on both normal and pathological ophthalmic tissues, we show that while the static FFOCT images better reveal the relative stationary cellular structures like nerve fibers and collagens, the dynamic FFOCT images show enhanced contrast of various transparent cells with active intracellular metabolic motions, offering complementary information of major corneal and retinal layers. Our study has shown the dual-mode FFOCT system is a straightforward promising technique for cellular imaging exploration and pathological analysis of ophthalmic tissues.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.