User-friendly photodamage-monitored imaging by simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy

Geng Wang; Rishyashring R. Iyer; Janet E. Sorrells; Kayvan F. Tehrani; Kajari Bera; Eric J. Chaney; Haohua Tu; Stephen A. Boppart

doi:10.1117/12.2608849

7 March 2022 User-friendly photodamage-monitored imaging by simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy

Geng Wang, Rishyashring R. Iyer, Janet E. Sorrells, Kayvan F. Tehrani, Kajari Bera, Eric J. Chaney, Haohua Tu, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC11954, Optical Biopsy XX: Toward Real-Time Spectroscopic Imaging and Diagnosis; PC1195404 (2022) https://doi.org/10.1117/12.2608849
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the low imaging speed and the accompanying photodamage risk hinder further clinical applications. To reduce these adverse effects, in this study, we constructed a new generation of simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy, featuring high-speed, multimodal imaging, monitorable-photodamage, and tunable ultrashort pulses. By using birefringent photonic crystal fiber and a pulse shaper, this system has the ability to allow users to independently adjust repetition rate, pulse width, and average power without overlapping interference, and can realize multiphoton generation in each channel from a single excitation pulse. These outstanding advantages represent a powerful and user-friendly imaging platform.

Conference Presentation

Citation Download Citation

Geng Wang, Rishyashring R. Iyer, Janet E. Sorrells, Kayvan F. Tehrani, Kajari Bera, Eric J. Chaney, Haohua Tu, and Stephen A. Boppart "User-friendly photodamage-monitored imaging by simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy", Proc. SPIE PC11954, Optical Biopsy XX: Toward Real-Time Spectroscopic Imaging and Diagnosis, PC1195404 (7 March 2022); https://doi.org/10.1117/12.2608849

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KEYWORDS

Microscopy

Imaging systems

Signal to noise ratio

Image processing

Image resolution

Optical microscopy

Photonic crystal fibers

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