In the realm of food safety, the standard practice involves collecting food product samples and sending them to a central laboratory for microbiological testing. However, this process introduces delays in obtaining the microbiological testing results and subsequently affects the timely delivery of food products to consumers. To further reduce the time-to-detection issue, we propose the development of a self-contained, battery-operated, high-sensitivity optical sensor that can be affixed to the cap of the typical food sample collection container. This device, called MPACT, offers real-time and in-transit monitoring of the contamination status of the food sample, specifically targeting E. coli O157:H7, through a bioluminescence assay. The assay exclusively targets the target pathogen and, when detected, produces minimal luminescence. As the sample is transported in the container, the number of bacterial cells multiplies, and once the luminescent signal reaches a predefined threshold, the sensor reports the results via Bluetooth. This study focuses on the design of the bottle cap sensor and examines its sensitivity by subjecting it to bioluminescence samples.
With the development and expansion of the internet of things, many scientific and engineering instruments are leaving the benchtop restriction and moving on to provide on-site detection. On-site detection requires a complete miniaturization of a benchtop system while maintaining a similar performance with respect to the analyte detection sensitivity. In addition, due to the mobile nature, utilizing a battery source is required. Here we present a portable loop-medicated isothermal amplification detection system for on-site detection and amplification of target analyte via fluorescence detection. The digital twin design incorporates three major components: an isothermal heating chamber, light-tight enclosure for sample insert, and fluorescence imaging system via micro-controllers. The isothermal heating chamber was designed with Peltier heater to provide small form factor accurate temperature control. For light-tight enclosure is a 3D printed device that allows DNA samples to be inserted and fluorescent images to be taken within the chamber. Lastly, fluorescent imaging system operates with a stand-alone camera connected to an Arduino micro-controller. Excitation is provided by blue colored LED and emission is detected via long-pass filter that matches the emission spectrum.
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