We present an acoustic-driven quantitative phase microscope for quantifying cell biomechanics at sub-cellular level. Longitudinal acoustic standing wave is established as mechanical loadings to deform cells. A reflection quantitative phase microscopy with high axial and lateral resolution is used to quantify the cell deformations. We evaluate cancerous and normal cells undergoing standing wave modulation, where both the time-lapse cell membrane fluctuations and whole-cell volumetric shape changes are measured. Our technique can achieve high throughput, label-free and non-invasive quantification of cell mechanics with high mechanical sensitivity over broad mechanical frequency response range and can potentially be extended to study 3D tissue mechanics with sub-cellular resolution.
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