The paper presents a structural and logical diagram and an analytical description of the differential diagnosis of aseptic and septic loosening of the cup of an artificial hip joint endoprosthesis using spectral-selective laser autofluorescence microscopy of coordinate distribution maps (MIF) and correlation (MCF) fluorescence intensity of polycrystalline films of synovial fluid (SF). The results of the statistical analysis [1-5] of the distributions of the MIF and MCF values of polycrystalline SF films of patients from the control group and groups with different severity of pathology of the hip joint are presented. The sensitivity, specificity and accuracy [6-9] of the method of spectral-selective laser autofluorescence microscopy MIF and MCF of the fluorescence intensity of polycrystalline films of hip joint CF have been established.
The structural-logical diagram and research design by the methods of polarization-phase tomography of linear dichroism of the polycrystalline structure [1-5] of histological sections of the brain are presented. Differential diagnosis of the formation of hemorrhages of traumatic genesis, cerebral infarction of ischemic and hemorrhagic genesis by the method of differential Mueller-matrix mapping of amplitude anisotropy - linear dichroism maps (ALD) of histological brain sections and operational characteristics of the method of their statistical analysis. Differential diagnosis of the prescription of the formation of hemorrhages of traumatic genesis, cerebral infarction, ischemic and hemorrhagic genesis by the method of differential Mueller-matrix mapping of amplitude anisotropy - temporal dynamics of changes in the statistical structure of ALD maps of histological brain sections.
The article presents the results of a study of the possibilities of 3D Stokes-polarimetric mapping of microscopic images of protein fluorofors of the prostate. Polarization-holographic measurement and analysis of layer-by-layer maps and histograms of the distribution of the polarization ellipticity of microscopic images of biological preparations of the prostate. Determination of the relationship between statistical moments of the 1st - 4th orders, which characterize layer-by-layer maps of distributions of the polarization ellipticity of microscopic images of biological preparations of the prostate and pathological conditions of the prostate. Determination of the operational characteristics (sensitivity, specificity, accuracy) of the diagnostic strength of the 3D layer-by-layer Stokes polarimetric mapping method.
The article presents the results of algorithmic approbation of digital histological research methods of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue on the basis of polarization reconstruction of linear birefringence maps of protein fluorophores of fibrillar networks; determining the relationship between the temporal change in the magnitude of the statistical moments of the 1st - 4th orders, characterizing the distribution of the magnitude of the degree of crystallization of histological sections of the brain, liver and kidney, as well as the myocardium and lung tissue, and the duration of damage; establishment of time intervals and accuracy of determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction (tomography) the degree of crystallization of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The materials of the experimental determination of the set of maps and histograms of the distribution of the magnitude of the fluctuations of linear (DFLB) birefringence for differential diagnosis and the formation of hemorrhages of traumatic origin, cerebral infarction ischemic and hemorrhagic genesis by diffuse tomography of the polycrystalline structure of histological sections of the brain.
For a high-precision objective histological determination of the prescription of damage to internal organs over a long period of time, a systematic approach was used based on digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods for studying temporary changes in the molecular and polycrystalline structure of brain, liver and kidney samples in the post-mortal period. It was revealed that a linear change in the magnitude of statistical moments of the 1st - 4th orders characterizing the distribution of data of digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods is interconnected with the duration of damage to internal organs. On this basis, a new algorithm for digital histological determination of the prescription of the occurrence of damage is proposed. To determine the extent of damage, the method of azimuthally invariant polarization microscopy with different magnification of the image of histological sections of tissues of internal organs was used, which provided diagnostic relationships between changes in the magnitude of statistical moments of the 1st to 4th orders, which characterize the azimuth and elliptic polarization maps of digital microscopic images and time intervals of damage duration.
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